Neuroimaging of Movement Disorders

Abstract

1389 Objectives Cancer cell lines, established from either primary or metastatic sites of cancer patients, have contributed to understanding cancer biology and the selection/validation of imaging radiotracers. Cell lines with a shorter doubling time (DT) are expected to have more aggressive behavior. In the present study, we assessed correlations between the DTs of hematological malignant cell lines and the abundance of messages for Ki67, PCNA, TK1, thymidylate synthase (TYMS), HK1, or transporters (SLC29A1-2) Methods A NCI 60 exon dataset (interrogating over 1 million exon clusters within the known and predicted transcribed regions of the entire genome) was selected from NIH Gene Expression Omnibus repository, which contains 178 chips including 6 hematological malignant cell lines with triplicates for each: HL-60, RPMI-8226, K-562, SR, CCRF-CEM, and MOLT-4. Using Partek, commercial software, we obtained an average abundance of the transcripts of interest above. Correlations between the abundance of genes and NCI documented doubling times were determined by Pearson correlation testing (α=0.05 for a one-tailed test). Results The correlation coefficients r of HK1 message (phosphorylating FDG) vs. DTs was -0.87 (p= 0.01 Conclusions In these cell lines, HK1 message abundance appeared to have a stronger correlation to DTs of hematological malignant cancer cell lines than message levels for TK1, TYMS, and conventional proliferation markers Ki67 and PCNA. The correlation is negative, supporting higher HK1 abundance and shorter DT. Since message levels and protein levels can sometimes be disparate, our findings should be validated using protein quantitation and in vivo. Research Support NIH T32 training grant