Abstract
The aim of the study was to investigate the distribution and content of neuroglobin in the pyramidal neurons of the frontal and parietal cortex of white rats during simulated cholestasis.Material and methods. The study included 60 outbred white male rats weighed 200–250 g. Cholestasis was simulated by ligation of the common bile duct in the porta hepatis area (main group, n=30). Animals of the control group (n=30) were performed a false operation preserving physiological bile outflow. Sections of the frontal and parietal cortex of white rats were selected for investigation. The content and distribution of neuroglobin was detected immunohistochemically on paraffin sections using mouse monoclonal primary antibodies Anti- Neuroglobin antibody (Abcam). Cytophotometric analysis was used to perform quantitative assessment of the content of the studied molecular marker; after that statistical analysis was performed.Results. It was found that the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modified in a wave-like mode. After ligation of the common bile duct, the content of neuroglobin significantly decreased in 2, 10 and 45 days (minimum on the 10th day) in the frontal cortex and in 90 days in the parietal cortex, and increased in 5 and 20 days of the experiment (maximum on the 20th day).Conclusion. In cholestasis, the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modifies in waves: a decrease is observed on the 2nd, 10th, 45th day; an increase is observed on the 5th and 20th day; a recovery is observed on the 90th day.
Highlights
Cholestasis was simulated by ligation of the common bile duct in the porta hepatis area
Sections of the frontal and parietal cortex of white rats were selected for investigation
It was found that the content of neuroglobin in the pericarions of neurons of the frontal and parietal cortex modified in a wave-like mode
Summary
Цель исследования – изучить распределение и содержание нейроглобина в пирамидных нейронах лобной и теменной коры головного мозга белых крыс при экспериментальном холестазе. Для исследования брали участки лобной и теменной коры головного мозга белых крыс. Установлено, что после перевязки общего желчного протока содержание нейроглобина в перикарионах нейронов лобной и теменной коры головного мозга изменялось волнообразно. В лобной коре содержание нейроглобина значительно уменьшалось через 2, 10 и 45 сут (минимум на 10-е сут) после перевязки общего желчного протока, а в теменной коре и на 90-е сут, и увеличивалось на 5-е и 20-е сут эксперимента (максимум на 20-е сут). При холестазе содержание нейроглобина в перикарионах нейронов лобной и теменной коры мозга изменяется волнообразно: на 2-, 10-, 45-е – наблюдается уменьшение, на 5- и 20-е сут – увеличение его содержания, и к 90-м сут – восстановление
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
