NEUROGENOTOXICITY EFFECT OF DELTAMETHRIN AND AMELIORATIVE EFFECT OF VITAMIN E & GREEN TEA ON FEMALE RATS

Abstract

several studies have been implicated oxidative stress and genotoxicity as important mechanisms of toxic effects of pyrethriods in the present study. The beneficial effects of vitamin E and green tea extract were tested in protecting the deltamethrin (DM) induced oxidative stress, genotoxicity and alterations in some amino acid neurotransmitters in brain of adult female rats .Rats were exposed to 1mg/Kg b.wt .deltamethrin (1/10 LD50 ) alone or in combination with vitamin E and/or green tea extract .The antioxidant such as catalse enzyme, reduced glutathione content, lipid peroxidation content and ChE activity were analyzed in cerebrum and cerebellum of brain excised at the end of experiment (30 days). Results showed that genotoxic effect of deltamethrin was proved by SSCP of HRas and P53 genes .Amino acids transmitters were estimated in brain of exposed DM rats . Exposure to deltamethrin resulted in a significant decrease in the activities of catalase, ChE and concentration of reduced glutathione, while the LPO levels were significantly increased in different brain regions .Also, DM exposure increases significant changes in brain amino acids. It is Interestingly that the supplementation with vitamin E or green tea extract or both resulted reversed in the deltamethrin induced antioxidant enzymes and effect on genotoxicity .Combination of both green tea and vit. E appeared to be more effective in protection than each one alone .