Abstract
We identified a rare undifferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal differentiation. Interestingly, such Sox2+ S100− cells of the adult cerebellum expanded after adequate physiological stimuli in mice (exercise), and Sox2+ precursors acquired positivity for the neuronal marker NeuN over time and integrated into cellular networks. In human patients, SOX2+ S100− cells similarly increased in number after relevant pathological insults (infarcts), suggesting a similar expansion of cells that lack terminal glial differentiation.
Highlights
Neurogenesis within the adult mammalian brain is traditionally said to be restricted to a few discrete niches[1], many of which are known to bear precursor cells that express the transcription factor Sox[22, 3]
To identify potential stem cell niches within the adult cerebellum, we carefully investigated the expression pattern of Sox[2]
Apart from Bergmann glia cells that are characterized by the co-expression of differentiation markers like S100B, Blbp and Gfap, we found a subset of Sox2-expressing cells in the Purkinje cell layer (PCL) that lack expression of S100, i.e. Sox2+ S100− cells that makes up 18.4% of the entire Sox2+ population within the PCL (Fig. 1c–e)
Summary
Neurogenesis within the adult mammalian brain is traditionally said to be restricted to a few discrete niches[1], many of which are known to bear precursor cells that express the transcription factor Sox[22, 3]. Training of mice on the RotaRod system lead to a significant increase in the number of Sox2+ S100− cells within the PCL as compared to controls (m – Mann-Whitney-U test; SEM).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
