Abstract
While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease.
Highlights
While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution
Transient simulations revealed that a 100% concentration of stimulus reaches the larva head in
The microfluidic chip (NeuroExaminer) is part of a larger ensemble and embedded in a custom-made imaging chamber; Supplementary Fig. 1 provides an overview of the setup used in this study
Summary
While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, have not been combined to monitor whole-brain neural activity yet. We report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can provide insights into brain function in health and disease. We report an all-glass microfluidic device that, in contrast to the more commonly used polydimethylsiloxane (PDMS), does not exhibit solvent permeability[7] or autofluorescence[8], and is compatible with whole-brain in vivo imaging via light-sheet microscopy
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