Neurocalcin-immunopositive neurons in the rat sensory ganglia

Abstract

Distribution of neurocalcin, a calcium-binding protein having three EF hand motifs, in the rat sensory ganglia was demonstrated immunochemically and immunohistochemically. Immunoblot analysis of trigeminal, nodose and dorsal root ganglia homogenates revealed an immunoreactive band at approximately 24 kDa. Neurons labeled by the neurocalcin-antiserum represent 54%, 41% and 46% cells in the trigeminal, nodose and dorsal root ganglia, respectively. Size distribution of immunopositive cells showed a varying range. Most large cells (more than 80%) showed immunoreactivity in the trigeminal and dorsal root ganglia. A double immunofluorescent study was performed to determine the colocalization with calbindin D-28k or parvalbumin, which are both calcium-binding proteins. In the trigeminal and dorsal root ganglia, almost all calbindin- or parvalbumin-immunoreactive neurons showed neurocalcin-immunoreactivity, whereas approximately 30–40% neurocalcin-immunopositive cells had calbindin- or parvalbumin-immunoreactivity. In the nodose ganglia, parvalbumin showed localization similar to other ganglia, but about half the calbindin-immunoreactive neurons had neurocalcin-immunoreactivity. These studies show that neurocalcin-immunopositive neurons are widely distributed in the sensory ganglia and most calbindin- or parvalbumin-immunoreactive neurons also contain neurocalcin. In the sensory neurons, neurocalcin may have a significant role in calcium signaling.