Abstract
Aberrant protein expression within the hippocampus has recently been implicated in the pathogenesis of obesity-induced memory impairment. The objective of the current study was to search for specific memory-related factors in the hippocampus in obese rats. Sprague-Dawley (SD) rats were fed either a high-fat (HF) diet or normal-fat (NF) diet for 10 weeks to obtain the control (CON), diet-induced obese rats (DIO) and diet-resistant (DR) rats. D-galactose was injected subcutaneously for 10 weeks to establish model (MOD) rats with learning and memory impairment. After the hippocampus of the rats sampling, the proteome analysis was conducted using two-dimensional get electrophoresis (2-DE) combined with peptide mass fingerprinting (PMF). We found 15 differential proteins that expressed in the hippocampus in rats induced by HF diet from the 2-DE map. In addition, Neurocalcin-delta (NCALD) was nearly down-regulated in the DR rats compared with CON rats and MOD rats, which was further confirmed by Western blot, real-time PCR and ELISA results. Our data demonstrates that the differential memory-related proteins were a reflection of the HF diet, but not potential factors in obesity proneness or obesity resistance. Furthermore, NCALD is proved to be a potential hippocampus-memory related factor related to obesity.
Highlights
Aberrant protein expression within the hippocampus has recently been implicated in the pathogenesis of obesity-induced memory impairment
We reported the evidence of obesity-induced alterations in expression of memory-associated proteins in the hippocampus of obese rats, and offered a novel mechanism by which protein expression within the hippocampus is suppressed during obesity
2-D gel analysis At the end of week 10, leukemia-associated phosphoprotein p18 (Lap 18) and β-actin were down-regulated in the hippocampus of diet-induced obese rats (DIO) and DR rats compared with CON rats and MOD rats
Summary
Aberrant protein expression within the hippocampus has recently been implicated in the pathogenesis of obesity-induced memory impairment. Results: We found 15 differential proteins that expressed in the hippocampus in rats induced by HF diet from the 2-DE map. Neurocalcin-delta: a potential memory-related factor in hippocampus of obese rats induced by high-fat diet. @ 2017 Ma et al; licensee African Health Sciences African Health Sciences Vol 17 Issue 4, December, 2017 campus This result is consistent with our previous studies[16,17]. Aberrant protein expression within the hippocampus has recently been implicated in the pathogenesis of obesity-induced memory impairments. We reported the evidence of obesity-induced alterations in expression of memory-associated proteins in the hippocampus of obese rats, and offered a novel mechanism by which protein expression within the hippocampus is suppressed during obesity
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