Neurobehavioral study of prenatal exposure to hyperthermia combined with irradiation in mice.

Abstract

Thirty-two pregnant adult LACA mice were randomly assigned to one of five exposure groups: control, 38 degrees C, 42 degrees C, irradiation, and 42 degrees C + irradiation. Animals were exposed on gestation day 9 to either 38 degrees C waterbath for 5 min, 42 degrees C waterbath for 5 min, treatment with 0.5 Gy of 60Co gamma irradiation, or pretreatment at 42 degrees C waterbath for 5 min following by 0.5 Gy of 60CO gamma irradiation. On postnatal day 7, litters were reduced to a maximum of eight pups per litter, with equal members of male and female offspring whenever possible. A total of 216 pups were observed for the age of acquisition of four physiological landmarks (pinna detachment, incisor eruption, eye opening, testes descent), six developmental reflexes (surface righting, cliff avoidance, auditory startle, air righting, visual placing, hindlimb splaying), and examination of learning and memory function. These studies, at threshold exposure levels to hyperthermia and ionizing radiation, indicate there is no consistent significant additional postnatal effect when pregnant mice are exposed to both of these agents. These studies are important for our understanding of the relative long-range effects of prenatal exposure to hyperthermia and acute dose irradiation early in gestation on neurobehavioral teratogenesis.