Abstract
Intrabodies are recombinant antibody fragments produced inside of a living cell in a way to bind their antigen in that same cell, to interfere with its function. As they strictly work on the protein level, intrabodies allow to bypass disadvantages of gene-targeted knock-out animals and knockdown techniques (RNAi, miRNA and small molecule inhibitors). Recently, a regulated knock-down strength was illustrated in viable VCAM1 knockdown mice.
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