Network Pharmacology Study on the Mechanism of Qiang Huo-Yinchen Treating Diabetic Peripheral Neuropathy

Abstract

Objective: To explore the mechanism of Qiang huo-Yin chen in the treatment of diabetic peripheral neuropathy (DPN) based on network pharmacology. Methods Using traditional Chinese medicine system pharmacology analysis platform (TCMSP), oral bioavailability (OB) ≥30 %, drug-like (DL)≥0.18 as screening conditions, notopterygium-Yinchen compounds and related targets were obtained. DNP-related genes were obtained from the human gene database (GeneCards) and the online human Mendel genetic database (OMIM). The intersection genes between candidate compounds and DNP were obtained by online Wayne diagram analysis. The interaction network of intersection proteins was constructed by STRING 10.0 database. Gene ontology (GO) analysis and signal pathway enrichment analysis based on Kyoto Encyclopedia of Genes and Genes (KEGG) were performed on the intersection proteins through DAVID database. Cytoscape 3.8.2 software was used to construct the component-target-signaling pathway network of Radix Astragali- Radix Angelicae Sinensis in the treatment of diabetic nephropathy. Results: The constructed component-target network contained 23 active components, including cnidilin, quercetin, beta-sitosterol, isorhamnetin, and areapillin. HSP90AA1, BLC2, CASP3 among 63 targets, KEGG involves 28 pathways such as lipid and atherosclerosis, AGE-RAGE in diabetic complications, apoptosis, pathway signaling pathways of neurodegenerative diseases. Conclusion: Qianghuo-yinchen pair may act on HSP90AA1, BLC2, CASP3 and other targets through multiple components such as quercetin and β-sitosterol, and regulate multiple signaling pathways such as lipid, apoptosis, and neurodegenerative diseases, thereby playing a role in the treatment of DNP.