Network Pharmacology Study on Molecular Mechanisms of Zhishi Xiebai Guizhi Decoction in the Treatment of Coronary Heart Disease.

Hao Chen,Haoyang Li,Yujing Pan,Jin Gao,Zishuo Mi,Vahidreza Ostadmohammadi,Yuxi Zhao,Xiaodong Tan

doi:10.1155/2021/3574321

Abstract

Background Coronary heart disease is characterized by the formation of arterial plaque. If not taken seriously, it will cause serious consequences such as myocardial infarction and heart failure. Zhishi Xiebai Guizhi Decoction first appeared in “Synopsis of Prescriptions of the Golden Chamber” and is a representative prescription for the treatment of coronary heart disease. This study aims to explain the mechanism of Zhishi Xiebai Guizhi Decoction in the treatment of coronary heart disease through network pharmacology and clinical trials. Methods We first identified the core compounds of Zhishi Xiebai Guizhi Decoction and their potential targets through TCMSP. Then, We analyzed the molecular targets of Zhishi Xiebai Guizhi Decoction in coronary heart disease with OMIM and GeneCards databases. After the common targets were screened out, we manage to figure out the pathways of these target genes through STRING. Finally, we verify the treatment results in clinical trials. Results Through network pharmacology analysis, we discovered that several core compounds of Zhishi Xiebai Guizhi Decoction have anti-inflammatory effects and are of great significance to treatment of cardiovascular diseases. The mechanism may be closely related to PPARγ, inflammation, TNF signaling pathway, AMPK signaling pathway, and PI3K-Akt signaling pathway. Clinical trials have also proved the key role of inflammation. Conclusions Zhishi Xiebai Guizhi Decoction may play a role in treating coronary heart disease by activating PPARγ. TNF signaling pathway, AMPK signaling pathway, and PI3K-Akt signaling pathway are potential mechanisms as well. The application of network pharmacology can provide a novel method for the research of Chinese herbal medicine. We hope that Zhishi Xiebai Guizhi Decoction will be recognized as a complementary or alternative treatment for coronary heart disease.

Highlights

Coronary heart disease (CHD) is characterized by formation of arterial plaques which are mainly comprised of lipids, calcium, and inflammatory cells [1]. ese plaques narrow the lumen of coronary arteries leading to episodic or persistent angina
E obtained intersection target was used as the drug effect target, and Cytoscape3.8.2 was employed to construct the drug effect target-component interaction network (Figure 4). e network was analyzed to get its degree value and get the key drug effect target (Table 2)
Identification of Targets of Zhishi Xiebai Guizhi Decoction and CHD in Various Databases. e database retrieved 139 relevant targets of the active ingredient, and the active ingredient-target interaction network was constructed using Cytoscape 3.8.2 (Figure 2). rough keyword search, 1991 related targets of coronary heart disease were obtained in GeneCards database and OMIM database. e Venny diagram constructs the intersection of active ingredient-target and disease-target

Summary

Introduction

Coronary heart disease (CHD) is characterized by formation of arterial plaques which are mainly comprised of lipids, calcium, and inflammatory cells [1]. ese plaques narrow the lumen of coronary arteries leading to episodic or persistent angina. Zhishi Xiebai Guizhi Decoction first appeared in “Synopsis of Prescriptions of the Golden Chamber” and is a representative prescription for the treatment of coronary heart disease. Is study aims to explain the mechanism of Zhishi Xiebai Guizhi Decoction in the treatment of coronary heart disease through network pharmacology and clinical trials. Rough network pharmacology analysis, we discovered that several core compounds of Zhishi Xiebai Guizhi Decoction have anti-inflammatory effects and are of great significance to treatment of cardiovascular diseases. Zhishi Xiebai Guizhi Decoction may play a role in treating coronary heart disease by activating PPARc. TNF signaling pathway, AMPK signaling pathway, and PI3K-Akt signaling pathway are potential mechanisms as well. We hope that Zhishi Xiebai Guizhi Decoction will be recognized as a complementary or alternative treatment for coronary heart disease

Methods

Results

Conclusion