Network Pharmacology-Based Study on the Active Component and Mechanism of the Anti-Non-Invasive and Invasive Bladder Urothelial Carcinoma Effects of Zhuling Jisheng Decoction.

Chenyu Ma,Na Zhao,Min Deng,Yi Lu,Miao Cheng,Zhenya Pan,Jianlong Wang,Osamah Ibrahim Khalaf

doi:10.1155/2021/7667707

Abstract

Zhuling Jisheng decoction is employed for the treatment of bladder urothelial cancer in clinical practice of traditional Chinese medicine. However, there are few studies on its precise mechanism. For the antibladder cancer action of Zhuling Jisheng decoction, a network pharmacological technique was used to design a component/target/pathway molecular regulatory network. The TCMSP dataset was used to identify the chemical makeup of Zhuling Jisheng decoction, which was then analyzed and assessed for oral bioavailability and pharmacological similarity. The chemical composition of Zhuling Jisheng decoction was identified through the TCMSP database, and it was evaluated and screened based on oral bioavailability and drug similarity. The GEO database was searched for genes associated with urothelial bladder carcinoma, and gene targets associated with bladder urothelial cancer resistance were chosen by comparison. The function and linked pathways of the target genes were examined and screened using annotation, visualization, and a comprehensive discovery database. The impact of Zhuling Jisheng decoction on urothelial bladder cancer was studied using Cytoscape software to create a component/target/pathway network. Finally, 69 and 55 target genes were discovered for noninvasive bladder urothelial cancer and invasive bladder urothelial cancer, respectively. In noninvasive urothelial cancer, 118 pathways were highly enriched, including the TNF signaling pathway and the IL-17 signaling route. 103 pathways were highly enriched in invasive urothelial cancer, including the p53 signaling route, bladder cancer route, and calcium signaling route. There were 18 and 15 drug targets associated with noninvasive and invasive bladder urothelial carcinoma prognoses. Many signaling pathways directly act on tumours, and indirect pathways inhibit the development of bladder urothelial carcinoma. This research establishes a scientific foundation for further research into the framework of action of Zhuling Jisheng decoction in the therapy of bladder urothelial cancer.

Highlights

The most frequent malignant tumour in the urinary system is bladder cancer
The results show that 17 prompt ingredients of DCQD can be promoted by “regulating the PI3K/AKT signaling pathway” apoptosis and inhibition of pathological damage and inflammation to improve acute pancreatitis [16]
The potential compounds were identified for twenty-seven Zhuling Jisheng decoction (ZLJSD) (Table 1)

Summary

Introduction

The most frequent malignant tumour in the urinary system is bladder cancer. Men are three to four times as likely as women to get bladder cancer [2]. The most frequent urological malignancy of human bladder cancers, urothelial carcinoma of the bladder, accounts for more than 90% of all bladder cancers, divided into two categories focused on the clinical and morphological characteristics [3]. The majority of urothelial carcinomas constitute noninvasive papillary tumours (PTA stage). These tumours are typically highly differentiated, develop slightly without spreading widely, and have an excellent clinical prognosis, while the remaining one-third of urothelial carcinomas (staged pT1 and above) are invasive tumours with weak differentiation, a rapid rate of progression, and the capacity to metastasize. Bladder cancer has the feature of “three highs:” a high morbidity rate, a high postoperative recurrence rate, and a high fatality rate

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Discussion

Conclusion