Network Pharmacology-based Investigation and Experimental Discovery of Resveratrol’s Mechanism of Liver Ischemia Reperfusion-Induced Oxidative Stress Damage

Abstract

Background: This study was aimed to investigate the hepatoprotective effect of RSV against oxidative stress damage which we induced an experimental liver Ischemia-reperfusion (I/R) model in rats. Methods: Female albino rats were divided into three groups (n = 8). Contrast to the control group, 1 ml saline (0.09 % NaCl) was administered intraperitoneally two days before the operation and after reperfusion in the sham group. 1 ml of 50 mg/kg Resveratrol was administered to the treatment group until two days before the operation. In addition, a single dose of 1 ml of Resveratrol was re-administered after reperfusion. The duration of liver ischemia/reperfusion was determined as 30 minutes in all groups. At the end of this experiment, antioxidant enzyme (CAT, SOD) activities and the levels of malondialdehyde (MDA) were measured as spectrophotometric in liver tissues homogenates. Result: Our study, tissue injuries were observed in the ischemia/reperfusion and Sham groups (p less than 0.001). In this study, significant decreases were observed in tissue MDA levels in the treatment group compared to the sham and control groups (p less than 0.001). In addition, antioxidant enzyme levels (SOD. CAT) were significantly increased in the treatment group compared to the control and Sham groups. Liver tissue samples were examined for hepatocyte damage and histopathological grade of the groups were shown. In our study, tissue injuries were observed in the ischemia/reperfusion and Sham groups (p less than 0.001). Liver edema, necrosis and PMNL infiltration were found to be decreased in the treatment group compared to the control group. The association of resveratrol with oxidative damage was also determined by network pharmacology analysis.