Network Biology Approach to Identify the Active Ingredients, Potential Targets, and Signaling Pathways in Phellinus Baumii and Salvia Miltiorrhiza Bunge on Vascular Health (P15-025-19)

Abstract

ObjectivesIncreasing attention has been paid to a range of botanical food supplement that help to maintain vascular health. Multiple components in botanical foods are expected to be working in concert with various targets. In a previous our animal study, Phellinus baumii and Salvia miltiorrhiza Bunge (PS) ameliorated endothelial and vascular dysfunction in a platelet activation rat model. This study aimed to provide the components, target molecules, phenotypes, signaling pathways, and investigate the mechanism of PS on vascular health. MethodsNetwork biology analysis was based on the data from two clinical trials. The first clinical trial was performed in healthy subjects using high-fat-induced vascular dysfunction model. The second clinical trial was performed in healthy smokers. Differential markers obtained from clinical data, Affymetrix microarray, metabolomics, together with ingredient of PS, were mapped onto the network platform termed the context-oriented directed associations. A network of “component-target-phenotype-pathway” was constructed. ResultsThe resulting vascular health network demonstrates that the components of PS are linked various target molecules for adhesion molecule production, platelet activation, endothelial inflammation, vascular dilation, and mitochondrial metabolism and detoxification, implicated with various metabolic pathways. ConclusionsUsing network biology methods, this study revealed the components and their target molecules, phenotypes, signaling pathways and provided wider information to support the synergistic mechanisms of PS on vascular health. Funding SourcesThis research was funded by the Bio & Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science & ICT and the BK21PLUS of the National Research Foundation.