Net CO2 assimilation of cacao seedlings during periods of plant water deficit

Abstract

The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, but as Ψ dropped below approximately -0.8 and -1.0 MPa, A was reduced to less than 1.5 μmol CO2 m(-2) s(-1). The relation between g and A was highly significant and conformed to an asymptotic exponential model, with A approaching maximal values at stomatal conductances of 55-65 mmol H2O m(-2) s(-1). Net assimilation varied linearly (r=0.95) with transpiration, and the slope of the A-E relation (WUE) was approximately 3.0 μmol CO2 mmol(-1) H2O throughout the range of stomatal conductances observed. C i was insensitive to water stress, even though both g and A were strongly affected. Under the experimental conditions used here, mesophyll photosynthesis did not appear to control g through changes in C i. As stress intensified within each drying cycle, WUE of nonirrigated seedlings did not decline relative to that of controls even though CO2 and water vapor exchange rates underwent large displacements. The effect of seed source was highly significant for WUE, and the basis for observed differences among genotypes is discussed.