Nerve Suture Combined With ADSCs Injection Under Real-Time and Dynamic NIR-II Fluorescence Imaging in Peripheral Nerve Regeneration in vivo.

Shixian Dong,Mo Chen,Liang Ji,Yimeng Yang,Sijia Feng,Yuefeng Hao,Jian Zhang,Xing Yang,Yuzhou Chen,Jun Chen,Xiaotong Li,Yan Wo,Huizhu Li

doi:10.3389/fchem.2021.676928

Abstract

Peripheral nerve injury gives rise to devastating conditions including neural dysfunction, unbearable pain and even paralysis. The therapeutic effect of current treatment for peripheral nerve injury is unsatisfactory, resulting in slow nerve regeneration and incomplete recovery of neural function. In this study, nerve suture combined with ADSCs injection was adopted in rat model of sciatic nerve injury. Under real-time visualization of the injected cells with the guidance of NIR-II fluorescence imaging in vivo, a spatio-temporal map displaying cell migration from the proximal injection site (0 day post-injection) of the nerve to the sutured site (7 days post-injection), and then to the distal section (14 days post-injection) was demonstrated. Furthermore, the results of electromyography and mechanical pain threshold indicated nerve regeneration and functional recovery after the combined therapy. Therefore, in the current study, the observed ADSCs migration in vivo, electrophysiological examination results and pathological changes all provided robust evidence for the efficacy of the applied treatment. Our approach of nerve suture combined with ADSCs injection in treating peripheral nerve injury under real-time NIR-II imaging monitoring in vivo added novel insights into the treatment for peripheral nerve injury, thus further enhancing in-depth understanding of peripheral nerve regeneration and the mechanism behind.

Highlights

The incidence of peripheral nerve injury was ∼13 to 23 per 100,000 persons per year (Evans, 2001; Taylor et al, 2008; Asplund et al, 2009), the consequences of which might cause lifelong disorder and permanent disability
adipose stem cells (ADSCs) Imaging in Nerve Regeneration to realign damaged nerves, through approximation of the nerve stumps by nerve suture (Sulaiman and Gordon, 2000; Evans, 2001; Campbell, 2008)
The results suggested that PbS QDs had promising potential for tracking ADSCs in vivo

Summary

Introduction

The incidence of peripheral nerve injury was ∼13 to 23 per 100,000 persons per year (Evans, 2001; Taylor et al, 2008; Asplund et al, 2009), the consequences of which might cause lifelong disorder and permanent disability. The efficacy of pure nerve suture is hindered, failing to achieve sufficient tissue regeneration and full neurofunctional recovery of the injured nerve (Lin et al, 2013; Carriel et al, 2014; Gu et al, 2014). Adipose stem cells (ADSCs) have been extensively studied in the field of peripheral nerve regeneration (Zheng and Liu, 2019; Jahromi et al, 2020; Yamamoto et al, 2020; Zhou et al, 2020). The marriage of nerve suture and simultaneous ADSCs injection into one modality is expected to be a novel potential for optimizing the therapy of peripheral nerve injury (Sullivan et al, 2016)

Methods

Results

Conclusion