Nerve Growth Factor Levels in Seminal Plasma and Their Correlation with Madura Cattle Semen Analys

Abstract

Nerve Growth Factor (NGF) is one of the Neurotropin protein families. There is an evidence that NGF not only plays an important role in the nervous system but also plays an important role in the reproductive system. The objective of this study is to determine NGF levels in seminal plasma and their correlation with Madura cattle semen quality. Ten candidate of Madura bulls belonged to the Livestock Breeding and Animal Health Unit Pamekasan Madura were selected with the classification 3-5 years of age, healthy, normal genitalia, and good libido. Semen evaluation included sperm concentration, sperm motility, sperm viability, and sperm plasma membrane integrity. The NGF levels in the samples of seminal plasma were analyzed using indirect enzyme-linked immunosorbent assay (ELISA). The results of the study showed that the average of NGF levels in seminal plasma of ten Madura cattle were 0.29±0.15 ng/ml and it had a significant correlation with sperm motility (P <0.05) but did not have a significant correlation with sperm concentration, sperm viability, and plasma membrane integrity (P> 0.05). In conclusion, NGF levels in seminal plasma had a positive correlation with sperm motility, which are related to sperm fertility.