Nerve endings in human sympathetic ganglia.

Abstract

Forty-eight human sympathetic ganglia from 22 sympathectomies were examined ultrastructurally after one of three different fixations: (1) glutaraldehyde + osmium tetroxide, (2) glutaraldehyde + potassium dichromate + osmium tetroxide, or (3) potassium permanganate. Three different kinds of synapsing nerve ending could be identified after all fixation schedules. Type 1: "Cholinergic," containing small, agranular vesicles 40-60 nm in diameter (75% of all vesicles) and some large granular vesicles (100 nm in diameter). The number of type 1 profiles decreased with increasing age. Type 2: "Adrenergic," containing small granular vesicles 40-70 nm in diameter (over 90% of the vesicles). Type 3: "Nonadrenergic, noncholinergic," characterized by large opaque vesicles 80 -160 nm in diameter (over 50% of all vesicles). The frequencies of the types were counted after potassium permanganate fixation: type 1 - 71%, type 2 - 23%, and type 3 - 6%. In addition, at least two types of nerve profiles were observed which did not form synapses: (1) profiles entirely filled with mitochondria (mitochondrial accumulation), and (2) large nerve profiles full of different kinds of vesicles, myelin figures, and mitochondria (axonal dilatation). It is concluded that the sympathetic ganglion cells receive cholinergic and adrenergic innervation and also a third type of innervation, the possible peptidergic nature of which is discussed.