Nephroprotective Effect of Methanol Extract of Crassocephalum Crepidioides (Benth.) S. Moore (Ebolo) during Paracetamol- Induced toxicity in Wistar Rats

Abstract

The effect of Crassocephalum crepidioides (Benth.) S. Moore against Paracetamol (PCM)-induced oxidative stress was investigated. Wistar rats were divided into five groups of six rats. The control was group 1, Groups 2 and 3 were administered 250 mg/kg/bwt PCM and 300 mg/kg/bwt methanol extract of C. crepidioides leaves (MECL) respectively, group 4 and 5 were co-administered with 250 mg/kg/bwt PCM + 300 mg/kg/bwt MECL and 250 mg/kg/bwt PCM + 50 mg acetylcysteine (NAC) respectively for 2 weeks, following 1week pre-administration with 300 mg/kg/bwt MECL and 50mg NAC respectively. Kidney damage was measured by evaluating serum urea and creatinine, while antioxidant status was assessed by evaluating glutathione (GSH) level, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase activities. Lipid peroxidation (LPO) was determined from malondialdehyde (MDA) levels. PCM had no significant effect on serum urea and creatinine levels, but significantly decreased glutathione levels, SOD and catalase activities, while the activity of glutathione-S-transferase and level of malondialdehyde (MDA) was increased significantly. Co-administration with MECL or NAC reversed these effects. In conclusion, C. crepidioides. protects against PCM-induced oxidative stress in the Kidneys of Wistar rats.