Nephrolithiasis and uricosuria diagnostics in dogs

Abstract

Nephrolithiasis and hyperuricosuria is a common pathology in dogs of various breeds. The problem of breeding lies in the fact that the diagnostic methods used: X-ray examination, determination of lipid composition in the blood and the urogenital microbiome, do not allow early diagnostics in young dogs before the onset of clinical changes, as well as to identify animals carrying mutant alleles of disease candidate genes. We have developed a test system for nephrolithiasis and uricosuria diagnostics in dogs, including primers and allele-selective probes. A control plasmid solution was used as a matrix. The system is based on the use of the TaqMan method. This test system allows for early diagnostics of animals with mutant alleles to monitor the state of health during ontogenesis, as well as the exclusion of these dogs from breeding before their puberty.