Abstract
Neopterin (Np), a catabolic product of guanosine triphosphate (GTP), is synthesized by human macrophages and is an important indicator of cellular immune system activation. Np is associated with the activated cellular immune response and therefore this molecule is a potential biomarker for the diagnosis and follow-up of a wide variety of physiological conditions including cardiovascular and neurodegenerative diseases, autoimmune disorders and viral infections. Within the scope of this study, it was aimed to synthesize neopterin-imprinted cryogel columns (Np-MIPs), which can selectively recognize Np in human body fluids. Np-MIPs were synthesized via the cryo-polymerization in presence of Np as the template molecule and characterized by swelling test, polymerization yield calculations, Brunauer-Emmett-Teller (BET) measurements and Scanning Electron Microscopy (SEM). The surface area was measured as 22 m2/g. Adsorption and desorption of Np from aqueous solutions were investigated, and selectivity studies were performed against pterine and glucose molecules. Maximum Np adsorption on Np-MIP was found to be 249.2 μg/g and Np-MIPs can adsorb Np 1.09 and 3.84 times selective than glucose and pterin, respectively. It was demonstrated that Np-MIP columns can selectively adsorb Np from serum and urine, with the adsorption capacities of 36 μg/g and 38.2 μg/g Np-MIP, respectively.
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