Neonatal murine skin-derived cells transplanted using a mini-chamber model produce robust and normal hair

Abstract

Hair follicle reconstitution models are useful tools for investigating signalling and cytokines during hair follicle morphogenesis and cycling. The chamber model is one of the most established methods available for the study of hair follicle reconstitution and appears to be the most reproducible. However, the chamber model has several deficiencies: infection of skin wounds and subsequent animal death commonly occur, a large number of cells are required and only one chamber can be transplanted onto each animal. We modified these deficiencies by using a mini-chamber method, which has the advantages of having a high graft take rate, requiring fewer cells and allowing several mini-chambers to be transplanted onto each animal. In our study, cultured dermal cells at different passages (0 to high) lost the ability to reconstruct hair follicles, but dermal cells cultured overnight (12 h) retained this ability. Using the assay, newborn mice dermal cells that were freshly isolated and cultured overnight (12 h), as well as cultured dermal papilla cells from mice vibrissa follicles, all reconstructed hair follicles. However, cultured dermal papilla cells from human scalp follicles could not reconstruct hair follicles. Copyright © 2013 John Wiley & Sons, Ltd.