Abstract
Background
 Globally, 2.5 million neonates die each year from hypothermia. The World Health Organization (WHO) considers neonatal hypothermia as a decrease of axillary temperature in infants below 36.5° Celsius. New mothers, or the nearest caregivers, should be aware that hypothermia is a newborn danger sign, in order to apply best practices regarding neonatal hypothermia prevention to reduce subsequent mortality.
 Objective
 To assess mothers’ knowledge and practice of neonatal hypothermia at a selected provincial hospital in Rwanda.
 Methods
 A cross-sectional study design and convenience sampling method were used to select 161 mothers with living babies. Data were collected using a structured questionnaire. Descriptive and inferential statistics were used to analyze data.
 Results
 About 97% of mothers had inadequate knowledge, and the majority (83%) had inadequate neonatal hypothermia practices. Predictor variables for knowledge were ANC attendance (OR=1.884, 95% CI=1.675-2.565, p=0.042) and secondary educational level (OR=1.787, 95% CI=1.521-2.187, p=0.032). Practice was significantly associated with educational level (OR=1.787, 95% CI=1.521-2.187, p=0.032). 
 Conclusion
 There is a need to increase a mother's evidence-based practice of neonatal hypothermia. This condition unchecked leads to high-risk life-threatening complications. Prevention should emphasize on observed gaps to decrease deaths related to neonatal hypothermia.
 Rwanda J Med Health Sci 2020;3(2):152-166
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