Abstract

Apolipoproteins B and A-1 concentrations have been measured by radioimmunoassay in eluates of whole blood spotted on filter paper. The stability of apolipoproteins has been studied during a period of 2 months. Apolipoprotein levels are determined within 15 days after collection in order to avoid the significant decrease of B/A-1 ratio observed afterwards. The within-assay and between-assay CVs for middle concentrations are 6.4% and 6.8%, respectively, for apo B, and 8.2% and 13.5% for apo A-1. The coefficient of correlation between dried blood and plasma B/A-1 ratio is r 2 = 0.907 ( n=30). The apolipoprotein concentrations have been measured in 994 3-day-old newborns; the mean ± SD is 0.214±0.073 g/l (apo B), 0.495±0.147 g/l (apo A-1) and 0.453±0.164 (B/A-1 ratio). The apo B concentration is unaffected by birth weight or gestational age, but it is correlated with sex. Linear multivariate regression has been found for the apo A-1 concentration which is influenced by gestational age and by sex. Girls have higher levels than boys for both parameters ( P<0.0001 for apo B; P=0.0037 for apo A-1). This radioimmunoassay technique for apolipoproteins meets the practical requirements of a neonatal screening program for familial hypercholesterolemia.

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