Abstract
Symptoms typical of Neofabraea populi infection were identified from seven hybrid aspen (Populus tremula L. 2 P. tremuloides Michx.) stands in southern Finland. Morphology of the fungal isolates in vitro and ascospores released from apothecia on cankers was compared with reference isolates and the literature, respectively. A damage inventory was compiled in five stands. The percentages of healthy, diseased and dead stems per stand were calculated. The disease caused remarkable losses within stands (up to 53% mortality). Random amplified microsatellite markers were amplified from single-ascospore isolates, isolates from wood tissue and three reference isolates of N. populi. Both morphological and molecular analyses confirmed that the causal agent was N. populi, as suspected. The results imply that this fungus may become a major problem in hybrid aspen plantations in southern Finland.
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