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Abstract
A new natural plant constituent has been isolated from the root of red beet ( Beta vulgaris subsp. vulgaris var. conditiva) and identified as the orange-coloured water-soluble pigment 5- O-β- D-glucopyranosylneobetanidin (neobetanin). Its structure was elucidated and characterized by 1H and 13CNMR spectroscopy, FAB mass spectrometry, absorption spectroscopy, enzymatic determination of the glucose moiety, high-performance liquid chromatography and thin-layer electrophoresis. 1H nuclear Overhauser enhancement spectra unambiguously identified the position of attachment of the sugar residue. Neobetanin was also found to occur as a minor constituent in petals of Opuntia ficusindica, Portulaca grandiflora, Zygocactus truncatus and in fruits of Phytolacca bogotensis and Rhipsalis warmingiana.
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