Neoangiogenesis in the Econiche of Opisthorchis Felineus After Partial Hepatectomy Accompanied by Superinvasive Opisthorchiasis

Abstract

Research goal: revealing the liver vessels formation mechanisms after partial hepatectomy accompanied by superinvasive opisthorchiasis (SO).Material and methods. The study was carried out on Syrian hamsters, weighing 98.0–110.0 g, mature animals of both genders. The animals were divided into three groups. Group I (n=168): a model of SO. Group II (n=40): partial liver resection in healthy animals. Group III (n=86): partial hepatectomy accompanied by SO. The histological specimens were stained with Mayer's hematoxylin and eosin by the methods of Van Gieson, Slinchenko, V.V. Kupriyanov. Immunohistochemical reactions were performed using primary antibodies to CD31, CD34, CD117, Oct4, and α-fetoprotein. The results were statistically processed, the differences were considered significant at p<0.05.Results. In animals of group I accompanied by SO, proliferation of progenitor cells (CD31+, CD34+, CD117+, Oct4+) was observed in the portal tracts and perivascular space with the development of vascular formations, cholangiocellular and hepatocellular differnеntiations. Liver regeneration in healthy hamsters during 1– 3 days consisted in an active reaction of mitotic and amitotic division of hepatocytes with the development of avascular islets. In the subsequent periods (the 7th day), the cells of preexisting vascular structures (sinusoids, capillaries) multiplied. After partial hepatectomy accompanied by SO (group III), there was an intensive proliferation of stem and progenitor cells with differentiation into endothelial cells, elements of cholangio- and hepatocellular differnеntiations; endotheliocytes and capillary pericytes multiplied.Conclusion. Neoangiogenesis during regeneration after partial hepatectomy in healthy animals is implemented like angiogenesis; in case of partial liver resection accompanied by SO, it is followed by the vessels formation mainly from progenitor cells (vasculogenesis), advancing the development of elements of cholangio- and hepatocellular differnеntiations.