Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Abstract

Indigofera zollingeriana is an important protein rich
 forage   and dye plant species that grows
 widely in Indonesia. It is difficult to propagate the plant through seeds due
 to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple
 shoot regeneration of the plant under in
 vitro conditions using different combinations of   BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node
 explants have high potential to regenerate with maximum number of regenerated
 shoots noted on the epigeal and the cotyledon node explants
 using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP +
 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations
 of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective
 manner. The rooted plants were easily acclimatized to external conditions in an
 environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate
 under in vitro conditions. This research
 will aid in future studies related to breeding, propagation, biosynthesis
 and extraction of active
 compounds.