Abstract
1. 1. Biochemically active mitochondria have been isolated from the free-living nematode, Turbatrix aceti. Electron photomicrographs indicate that the preparation consists mostly of intact organelles. 2. 2. The mitochondria oxidized all of the tricarboxylic acid metabolites tested, as well as NADH. α-Phosphoglycerol, dihydroxyacetone, and palmityl-cocnzvme A were inactive. 3. 3. The mitochondria contain a functioning cytochrome system. Cytochrome a- a 2, appears to be the terminal oxidase. 4. 4. Spectra of oxidized and reduced cytochromes are reported. 5. 5. An additional pigment is present in the mitochondria in 6-fold greater quantity than the other cytochromes. The material binds CO, and probably CN −. It is reduced by dithionite and, in the presence of CO, by succinatc or NADH. The function of this pigment is unknown. It does not appear to be a terminal oxidase.
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