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Abstract
The root extract of Heracleum candicans Wall. exhibited antagonistic activities against nematodes Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle and Panagrellus redivivus (Linn.) Goodey. Through bioassay-guided fractionations, three coumarins were obtained from the extract of H. candicans and determined to be 8-geranyloxypsoralen (1), imperatorin (2), and heraclenin (3) based on spectra data. All three compounds possessed nematicidal activities against the two tested nematodes. The median lethal concentrations (LC50) of compounds 1–3 at 72 h were 188.3, 161.7, and 114.7 mg L−1 respectively against B. xylophilus and were 117.5, 179.0, and 148.7 mg L−1 respectively against P. redivivus. This is the first report about species in the Umbelliferae family that possesses nematicidal activity.
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