Abstract
Considering foremost global issues instigated by parasitic nematodes, Solanum nigrum (S. nigrum) and Mentha arvensis (M. arvensis) nematicidal potential at the gene level has been explored herein. Methanol, ethyl acetate, chloroform, n-hexane, and distilled water were used for extract preparation. Caenorhabditis elegans (C. elegans) was used as the model organism. Nematicidal and anti-egg hatching assays, fluorescence microscopy, and quantitative real-time PCR were done. S. nigrum chloroform (LD50 = 1.21 mg/mL) and M. arvensis methanol (LD50 = 2.47 mg/mL) extracts exhibited excellent nematicidal potential. Both plants showed potent anti-egg hatching activity (1 mg/mL). S. nigrum methanol and M. arvensis ethyl acetate extracts showed high apoptotic effect in muscles, gonads, and uterus (eggs). Stress genes, that is, gst-4, hsp-16.2, and gpdh-1 were highly expressed in affected C. elegans (treated with S. nigrum and M. arvensis leaf extracts) when compared with normal C. elegans. Phytochemicals and bioactive compounds present in plants may be the major cause of their excellent nematicidal potential, which further confirmed that both plants could be an alternative candidate(s) for novel broad-scale anthelmintic drug(s).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
