Nematicidal activity of aqueous tinctures of medicinal plants against larvae of the nematodes Strongyloides papillosus and Haemonchus contortus

A M Kabar,O O Boyko,V V Brygadyrenko

doi:10.15421/012016

Abstract

The study focuses on in vitro effect of aqueous tinctures of 48 species of herbaceous, shrub and tree plants on the first-third stage larvae of Strongyloides papillosus (Wedl, 1856) and third-stage larvae of Haemonchus contortus (Rudolphi, 1803) Cobb, 1898. The highest level of the effect was exerted by 3% aqueous tinctures of Wisteria sinensis (Sims) DC., Ailanthus altissima (Mill.) Swingle, Laburnum anagyroides Medik., Quercus petraea subsp. iberica (Steven ex M. Bieb.) Krassiln., Ginkgo biloba L., Colchicum autumnale L., Aristolochia manshuriensis Kom., Celastrus scandens L., Securigera varia (L.) Lassen, Magnolia kobus DC. Over 90% of the first and second non-invasive stage larvae of S. papillosus died at contact with these tinctures. The lowest parameters of LD50 were seen for L. anagyroides, Juniperus sabina L., C. scandens, M. kobus, A. manshuriensis, Wisteria sinensis (Sims) DC. and Securigera varia (L.) Lassen. Invasive larvae of S. papillosus and H. contortus were resistant to the effect of all the 48 surveyed species of plants. Third-stage larvae of H. contortus remained vital when exposed for 24 h to all the studied concentrations up to 3% aqueous tincture of plants. The results of the experiments and also the analysis of the literature indicate the necessity to continue the survey on nematocidial activity of aqueous tinctures and alcveshol extracts of plants.

Highlights

The health of agricultural animals, including the intensity of their infection with parasites, depends on the conditions of their maintenance
Wisteria sinensis (Sims) DC. is one of the plants we studied in 3%
Invasive larvae of S. papillosus and H. contortus in in vitro experiments were resistant to 3% aqueous tincture of the studied plants

Summary

Introduction

The health of agricultural animals, including the intensity of their infection with parasites, depends on the conditions of their maintenance. None of the separate factors in themselves has a great effect, for it is their mutual impact on parasites which is important. A significant role is played by the species composition of the parasitofauna, relations between all species of the pasture community, including agricultural animals, earthworms, mollusks, Acari and insects – the inhabitants of the pastures’ soils and feces of mammals, and various species of meadow grasses. A relevant aspect is the effect of the preparations or substances of non-synergic origin (Boyko & Brygadyrenko, 2018, 2019а), medicinal and other species of plants on the survival of parasites in the organism of the host in the environment (Boyko & Brygadyrenko, 2019b)

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