Abstract
BackgroundThe aim of this work was to investigate the potential nematicidal activity of Annona crassiflora leaf extract against Caenorhabditis elegans.MethodsThe hydroalcoholic leaf extract and its fractions (dichloromethane, ethyl acetate, methanol and water) were submitted to mobility assay against the roundworm Caenorhabditis elegans. GC-MS and NMR analysis were performed in order to identify metabolites.ResultsThe dichloromethane and ethyl acetate fractions showed to be the most active among the hydroalcoholic leaf extracts and its four fractions. The percentages of C. elegans larvae immobility were 98.13 and 89.66%, respectively, at a concentration of 1000 μg.mL−1. Besides some amino acids, palmitic acid methyl ester, 2-isopropyl-5-methylcyclohexanol, oleic acid methyl esther, stearic acid methyl ester, quercetin and kaempferol were also identified in these fractions.ConclusionThe results indicated that of A. crassiflora leaf ethanolic extract has a good potential as a source for natural nematicide.
Highlights
The aim of this work was to investigate the potential nematicidal activity of Annona crassiflora leaf extract against Caenorhabditis elegans
Hydroalcoholic extract activity was observed in the Caenorhabditis elegans mobility test, (Table 2)
As trigonelline was identified in the hydroalcoholic extract, and it is well known that trigonelline plays an important role in the resistance process of plants against several pathogens [19], commercial standard trigonelline was tested in the same assay
Summary
The aim of this work was to investigate the potential nematicidal activity of Annona crassiflora leaf extract against Caenorhabditis elegans. Parasitism attributed to nematodes is a worldwide problem that can negatively affect livestock, human health and plant growth. In animal breeding it can result in stunted growth, lower herd weight gain and can negatively influence meat quality. The most widespread form of nematode control is that performed with synthetic anthelmintics Their improper use has favored the selection of resistant strains, besides being toxic and impactful to the environment [4]. In the last decade there has been an increasing search for new methods of controlling animal and plant parasitic nematodes, using methods less toxic to the environment and to people [3]. The search for compounds from natural sources stands out
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