İneklerin Süt, Meme Başı Derisi ve Burun Mukozalarından İzole Edilen Stafilokok Türlerinin Antibiyotik Direnç Profilleri

Abstract

Summary The purposes of this study were to determine antibiotic resistance patterns of Staphylococcus spp. from isolated from milks, swabs from teat skins and noses mucosas of bovine in small and middle scale dairy farms. The samples were obtained from 109 head cows, randomly selected, reared in 14 small scale and 5 medium scale dairy farms in Kirikkale province. Isolation and identification of Staphylococci was made using biochemical tests and commercial micromethods (Crystal Gram-Positive Identification Kit, Becton Dickinson,USA). Resistance of Staphylococci spp. to various antibiotics were tested using disc diffusion test. Methicillin resistance of Staphylococci were determined using cefoxitin disc and oxacillin agar. Creature of β-lactamase enzyme was detected using β-lactamase (nitrocefin) identification stics (Oxoid). Forty-eight percent Staphylococcus aureus were isolated from bovine's milk samples in both small and medium scale dairy farms . S. aureus were isolated from in bovine's teat skin and noses mucosa swabs samples in small scale dairy farms, while S. aureus were not isolated from those of in middle scale dairy farms. It was applied to determine resistance by using 11 antibiotics which were blank to different antibiotic groups. Although the levels of resistance differed in origin of bovine' samples (noses mocosa- milk, between 19.5-49.5%), the highest percentage for resistance was obtained for penicillin. Methicillin resistant S. aureus was not isolated in this study. Of penicillin resistant Staphylococci, 92.7% (12) were found to be β-lactamase positive.