Abstract
Fission yeast wee1 − mutants initiate mitosis at half the cell size of wild type. The wee1 + activity is required to prevent lethal premature mitosis in cells that overproduce the mitotic inducer cdc25 +. This lethal phenotype was used to clone wee1 + by complementation. When wee1 + expression is increased, mitosis is delayed until cells grow to a larger size. Thus wee1 + functions as a dose-dependent inhibitor of mitosis, the first such element to be specifically identified and cloned. The carboxy-terminal region of the predicted 112 kd wee1 + protein contains protein kinase consensus sequences, suggesting that negative regulation of mitosis involves protein phosphorylation. Genetic evidence indicates that wee1 + and cdc25 + compete in a control system regulating the cdc2 + protein kinase, which is required for mitotic initiation.
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