Abstract
The existence of adult stem cells in the midgut and hindgut of Drosophila has been reported. The components of Hippo pathway such as dWarts and dYki were involved in the regulation of stem cell proliferation in the midgut. In this study, we reported the role of dMats in the control of stem cell proliferation in the midgut and hindgut of adult fruit fly. The eGFP signals driven by dmats indicated that dMats expressed in small diploid cells in the adult midgut and hindgut. By inducing MARCM cell clones, the dmats -/-cell clones in the midgut exhibited a bigger cell number than wild type cell clones and tissue outgrowth. Similarly, the dmats-/- mutation also caused more cell numbers in hindgut. Thus, dMats was critical to control stem cell proliferation in midgut as well as in hindgut. Our results implicated an important regulatory role for Hippo pathway in the regulation of adult stem cell proliferation.
Highlights
In the adult stage of organism, many types of tissues are wore out or damaged due to physiological or pathological situation, new cells need to be generated to replenish the lost tissues
In order to clarify the functions of dMats in the adult fly, we initially examined its expression in the adult fly gut, since the adult stem cells were found to reside in the epithelial tissue of gut (Ohlstein et al 2006; Micchelli et al 2006; Ohlstein et al 2007; Takashima et al, 2008). dmats driving eGFP strain (w; p[gmats]eGFP) was used to study the expression of dMats
As one type of somatic stem cell, epithelial stem cell is very important in the stem cell research since epithelial tissue protects the organs and it is replenished during all the life span of any organisms
Summary
In the adult stage of organism, many types of tissues are wore out or damaged due to physiological or pathological situation, new cells need to be generated to replenish the lost tissues Those cells that possess the ability to renew through mitotic dividing and the ability to differentiate into several different types of cells are adult stem cells. DMats, one of the key components, is a scaffold protein activating dWarts kinase activity, which in turn phosphorylats transcription co-activation dYki to inhibit cell number increase (Lai et al 2005; Wei et al 2007; Shimizu et al 2008) It is largely unknown if the dMats could regulate stem cell in midgut. The role of dMats in the ISC proliferation was examined and our evidence showed that loss-of-function of dMats increased cell proliferation of ISC residing in both midgut and hindgut
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