Abstract
Previously, we reported the strong negative interference of calcium dobesilate, a vasoprotective agent, in creatinine assays involving the Trinder reaction. It is hypothesized that a similar effect occurs in the detection of uric acid (UA), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). The interferences of calcium dobesilate during the detection of the five serum analytes were investigated on automated systems/analysers, and the effects were compared among eight different assay systems for each analyte. A calcium dobesilate standard was added into two sets of the blank serum pools of each analyte at final concentrations of 0, 2, 4, 8, 16, 32, and 64 μg/mL. The percentage deviation of each analyte value was calculated between each drug concentration and the drug-free samples. The clinically acceptable error levels for UA, TC, TG, HDL-C, and LDL-C were defined as ±4.87%, ±4.1%, ±9.57%, ±5.61%, and ±5.46%, respectively. The observed interference was concentration dependent for each analyte. In the presence of 16 μg/mL calcium dobesilate, which was within the therapeutic range, all seven Trinder reaction-based UA assay systems, two TG assay systems, two HDL-C assay systems and one TC assay system exhibited negative drug interferences. Calcium dobesilate negatively interferes with the detection of UA, TG, TC, and HDL-C in assay systems based on the Trinder reaction. The effect was most significant in UA and TG detection.
Highlights
The Trinder reaction is a fundamental process employed in a variety of clinical biochemical tests
We evaluated the interference of calcium dobesilate in different assay systems for the detection of uric acid (UA), total cholesterol (TC), TG, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) using an in vitro addition method
We confirmed through in vitro addition experiments that calcium dobesilate negatively interferes with the detection of UA, TC, TG, and HDL-C in assay systems based on the Trinder reaction
Summary
The Trinder reaction is a fundamental process employed in a variety of clinical biochemical tests. Trinder-coupled chromogenic reactions are used for the determination of creatinine (Cr), uric acid (UA), cholesterol, and triglycerides (TG). Phenol was used as the chromogenic agent for the Trinder reaction. With the application of a number of alternative chromogenic materials, such as 2,4-dichlorophenol, the sensitivity of this method has been improved significantly. Due to the poor substrate specificity of catalytic.
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