Abstract
The content of proenkephalin messenger RNA (PEmRNA) in cultured bovine adrenal chromaffin cells was reduced in the presence of reserpine (1 nM to 0.1 μM) with a return to basal levels 3 days after removal of the drug. In these cells, the basal release of Met 5-enkephalin-Arg 6-Phe 7 immunoreactivity (MERF-IR) into the medium was significantly decreased when the cultures were pretreated with 0.2 μM reserpine for 3 days. The addition of 0.1 μM etorphine for 3 days also decreased the basal release of MERF-IR without depleting stores of catecholamines. Neither drug modified the total (cells + medium) amount of MERF-IR. In contrast, reserpine was without effect on levels of PEmRNA or release of Met 5-enkephalin immunoreactivity (ME-IR) in primary cultures of the striatum of the fetal rat. The present data establish a correlation between inhibition of the secretion of enkephalin and reduced accumulation of its specific mRNA, suggesting a negative feedback inhibition by low molecular weight enkephalins.
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