Negative carry-over effects on larval thermal tolerances across a natural thermal gradient.

Abstract

Under climate change, marine organisms will need to tolerate or adapt to increasing temperatures to persist. The ability of populations to cope with thermal stress may be influenced by conditions experienced by parents, by both genetic changes and transgenerational phenotypic plasticity through epigenetics or maternal provisioning. In organisms with complex life cycles, larval stages are particularly vulnerable to stress. Positive parental carry-over effects occur if more stressful parental environments yield more tolerant offspring while the opposite pattern leads to negative carry-over effects. This study evaluated the role of parental effects in determining larval thermal tolerances for the intertidal mussel, Mytilus californianus. We tested whether thermal environments across a natural gradient (shoreline elevation) impacted mussel temperature tolerances. Lethal thermal limits were compared for field-collected adults and their larvae. We observed parental effects across one generation, in which adult mussels exposed to warmer habitats yielded less tolerant offspring. Interestingly, although parental environments influenced offspring tolerances, we found no clear effects of habitat conditions on adult phenotypes (tolerances). We found indicators of trade-offs in energy investment, with higher reproductive condition and larger egg diameters in low stress environments. These results suggest that parental effects are negative, leading to possible adverse effects of thermal stress on the next generation.