Neem leaf glucosamine stimulates Interleukin-2 (IL-2) in swiss albino mice

Santhosh Kumar Venugopalan,Rajasekaran Aiyalu,Navaratnam Viswesharan,Nair N K

doi:10.1038/npre.2011.5923.1

Santhosh Kumar Venugopalan, Rajasekaran Aiyalu + Show 2 more

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https://doi.org/10.1038/npre.2011.5923.1

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Abstract

AbstractNeem leaf glucosamine-mediated immune stimulation was studied in male swiss albino mice. Mice were treated with three doses of Neem leaf glucosamine (266, 400 and 800 µg/30gm mouse, intraperitoneal injection) one dose per week for four weeks. At doses of 266, 400 and 800 µg/30gm mouse a significant increase in Interleukin-2 in mice serum was observed when compared to the control group and also an increase in relative organ weight of the thymus was observed but there was a mild increase in liver and kidney weights. The immune stimulatory response of Neem leaf glucosamine was also observed in T-lymphocyte proliferation. Overall, Neem leaf glucosamine showed a stimulatory effect on immune functions in mice. The immunostimulatory effect of Neem leaf glucosamine as reported in this study will have special relevance for the prophylactic and potential therapeutic treatment of various infectious diseases and antitumor activity.

Highlights

Three groups of Male Swiss Wistar mice were immunized with Neem leaf glucosamine at the dose of 266 μg/30 gm mouse, 400 μg/30 gm mouse and 800 μg/30 gm mouse weekly for 4 weeks. (Fig. 1; Table 1 and 2) show the IL-2 concentration in mice serum were significantly increased from the three different dose of the Neem leaf glucosamine (266 μgm/30gm mouse, 400 μgm/30gm mouse & 800 μgm/30gm mouse) treated group when compared to the control group
The increase in the dose of the Neem leaf glucosamine treated group significantly showed an increase in the Interleukin-2 (IL-2) concentration in mice serum when compared to the control group
We identified and characterized the active component of Aqueous Neem leaf extract that is responsible for immunostimulatory activity

Summary

Introduction

Juss.) is a sacred gift of nature, mainly cultivated in Indian subcontinent [1]. Neem is a member of the mahogany family, Meliaceae. Every part of this tree has been used as traditional Indian medicine for household remedy, against various human diseases [2]. In 1992, US National Academy of Science published a report entitled, ‘Neem-a tree for solving global problem’ [3]. The first recorded use of Neem is attributed to the ancient East Indian ‘Harrappa Culture’ which added the plant to dozens of health and beauty aids 4500 years ago

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