Need of adjustment of methanogenic activities from solids digester sludge: Modelling dilution effects on micro-organisms biomass concentration

Abstract

Anaerobic digestion is a widespread technology used for organic-based solid waste management. Specific methanogenic activity tests are simple and cost-effective tools for sludge characterization and system diagnosis. However, in solid digesters, substrate and organic inert material dilute micro-organisms, enlarging the activity tests and distorting experimental results. To correct this situation, correction factors, defined as the ratio of micro-organisms concentration to total volatile solids content, are considered. Due to the impossibility to have a quick measurement of the mass of micro-organisms in the digester content, correction factors were evaluated based on the simulation of a simple model. To verify the importance of the correction, hydrogenotrophic and acetoclastic methanogenic activities were studied, involving a stage for sludge acclimatization and subsequent processing in a continuous digester. This situation was compared with a wastewater up-flow anaerobic reactor where no dilution effects are presented. A correcting factor of 0.79 was obtained for the acclimatization period, whereas correcting factors of 0.25–0.30 were estimated for the two periods of the digester. Tendencies shown for raw activities differed from those observed after using correcting factors to adjust activity values for periods 1 and 2 of the digester; also, the gap between the up-flow anaerobic reactor (without solids dilution effects) and the digester activities was reduced from sixfold to double, evidencing the relevance of this correction tool. Additionally, correcting factors also enabled a reasonable calculation of the inoculum size during the design of the activity tests.