Nectary structure in Symphyglossum sanguineum (Rchb.f. ) Schltr. (Orchidaceae)

Małgorzata Stpiczyńska,Kevin L Davies

doi:10.5586/aa.2006.001

Abstract

Ornithophily occurs in a great number of orchid species but despite this, researchers have largely neglected to investigate their nectaries. The aim of this study is to describe the nectary structure of Symphyglossum sanguineum, a species presumed to be pollinated by hummingbirds. The nectary is located at the free margins of auricles, which form a channel for the passage of nectar. The nectary, which consists of a single-layered epidermis and 2-3 layers of subepidermal cells, is supplied by collateral, vascular bundles. The nectary cells of S. sanguineum, like those of other ornithophilous orchids, have thick cellulose cell walls. A remarkable feature of these nectary cells is the dissolution of the middle lamella and the subsequent separation of epidermal cells. It is possible that this latter process facilitates the flow of the nectar to the nectary surface. The cuticle covering the nectary epidermis has micro-channels, but unlike the other species of ornithophilous orchids studied to date, it neither becomes disrupted nor detached from the epidermal cells. Abundant mitochondria, lipid droplets and smooth endoplasmic reticulum (SER) with an osmiophilic material are present in the cytoplasm of nectary cells. Some plastids with few lamellae contain numerous vesicles and osmiophillic globules whereas others accumulate starch. SER lamellae are often closely associated with plastids and the contents of the former organelles closely resemble osmiophillic globules. Secretory vesicles are common, especially near the outer, tangential wall indicating that granulocrine secretion possibly occurs in S. sanguineum.

Highlights

The floral diversity of orchids reflects both the pollination strategies employed and the type of reward offered to pollinators
The aim of the present paper is to describe the nectary structure of the presumed ornithophilous orchid Symphyglossum sanguineum and comparison with that of H. imbricata and M. coccinea would perhaps help us better understand the structural features that characterize the nectary of ornithophilous orchids
Hand-cut sections through the nectary were tested for starch and Nectary structure in Symphyglossum sanguineum (Rchb.f.) Schltr. (Orchidaceae) lipids using IKI and a saturated alcoholic solution of Sudan III, respectively

Summary

Introduction

The floral diversity of orchids reflects both the pollination strategies employed and the type of reward offered to pollinators. The nectary is in the form of the spur that arises from one of the perianth segments as in Calanthe, Comparettia, Disperis, Satyrium and Tipularia (Dressler , 1990) or as an outgrowth from the proximal part of the labellum as in Platanthera (Stpiczyñska , 1997) or Gymnadenia (Stpiczyñska and Matusiewicz , 2001). In Hexisea imbricata and Systeloglossum, the nectary is represented by a saccate spur formed by the fusion of the column and proximal part of the labellum (Dressler , 1990; Stpiczyñska et al, 2005). The nectary structure and the manner in which nectar is secreted can be very diverse (Pais and Figueiredo, 1994; Stpiczyñska , 1997; Stpiczyñska and Matusiewicz , 2001; Stpiczyñska et al, 2004; 2005)

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