Abstract
Black flies were collected twice daily using a vehicle-mounted insect net on 8 sample days between 20 May and 9 July 1993. The contents of the crops and midguts of 160 male and 160 female Simulium venustum Say were analyzed individually by thin-layer chromatography. Fourteen different combinations were identified of the following sugars: fructose glucose sucrose/turanose, melezitose, raffinose, and stachyose. Presence of melezitose and stachyose indicated that black flies had fed on homopteran honeydew. Significantly more female (40.0%) than male flies (27.5%) fed on honeydew, and this difference was caused by a skewedness in the morning samples.
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