Nearly Complete Genome Sequences of Two Bovine Viral Diarrhea Virus Isolates, Subtype 1f Strain SLO/1170/2000 and Subtype 1d Strain SLO/2416/2002.

Ivan Toplak,Jože Grom,Urška Kuhar,Nataša Toplak,Danijela Rihtarič,Jelle Matthijnssens

doi:10.1128/mra.00931-19

Ivan Toplak, Jože Grom + Show 4 more

Open Access

https://doi.org/10.1128/mra.00931-19

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Abstract

Bovine viral diarrhea virus (BVDV) subtypes 1f and 1d were isolated for the first time in Slovenia in 1999 and detected later in a majority of BVDV-infected cattle herds. Here, we report the first nearly complete genome sequences of noncytopathogenic BVDV-1f strain SLO/1170/2000 and cytopathogenic BVDV-1d strain SLO/2416/2002, isolated in Slovenia.

Highlights

Bovine viral diarrhea virus (BVDV) subtypes 1f and 1d were isolated for the first time in Slovenia in 1999 and detected later in a majority of BVDV-infected cattle herds
For sequencing of the BVDV strain SLO/2416/2002 genome, the cDNA was synthesized with the cDNA synthesis system (Roche, Manheim, Germany) and fragmented using the Covaris M220 instrument, targeting peak fragment lengths of 400 bp
The library was prepared with the GeneRead DNA Library L core kit (Qiagen) and quantified with a GeneRead Library Quant kit (Qiagen) and a Qubit v.3.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA)

Summary

Introduction

Bovine viral diarrhea virus (BVDV) subtypes 1f and 1d were isolated for the first time in Slovenia in 1999 and detected later in a majority of BVDV-infected cattle herds. Total RNA extraction from supernatants of isolates was performed using the QIAamp viral RNA kit (Qiagen, Hilden, Germany). For sequencing of the BVDV strain SLO/1170/ 2000 genome, a previously described protocol was used [8].

Results

Conclusion