Abstract
This work describes the nearly complete genome sequence of Newcastle disease virus (NDV) strain NDV/Novosibirsk/garganey/27/2014, which was isolated from a wild garganey in western Siberia, Russia. The NDV strain was classified as belonging to class II of genotype I and was identified as having recent common ancestry with isolates from wild and domestic birds in China and South Korea.
Highlights
Virulent strains of Newcastle disease virus (NDV) cause economically significant disease in wild birds and poultry [1]
RNA was isolated from 100 l of chicken allantoic fluid with cultured viral particles using a GeneJET viral DNA/RNA purification kit (Thermo Fisher Scientific) and was treated with Turbo DNase (Thermo Fisher Scientific)
Up to 40 ng of RNA was used for the DNA library, which was prepared by using the TruSeq RNA sample preparation kit version 2, without using oligo(dT) beads (Illumina)
Summary
Virulent strains of Newcastle disease virus (NDV) cause economically significant disease in wild birds and poultry [1]. RNA was isolated from 100 l of chicken allantoic fluid with cultured viral particles using a GeneJET viral DNA/RNA purification kit (Thermo Fisher Scientific) and was treated with Turbo DNase (Thermo Fisher Scientific).
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