Near-Infrared Spectroscopy as an Analytical Process Technology for the On-Line Quantification of Water Precipitation Processes during Danhong Injection.

Xuesong Liu,Ye Jin,Yongjiang Wu,Chunyan Wu,Shu Geng,Lianjun Luan,Yong Chen

doi:10.1155/2015/313471

Xuesong Liu, Ye Jin + Show 5 more

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https://doi.org/10.1155/2015/313471

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Abstract

This paper used near-infrared (NIR) spectroscopy for the on-line quantitative monitoring of water precipitation during Danhong injection. For these NIR measurements, two fiber optic probes designed to transmit NIR radiation through a 2 mm flow cell were used to collect spectra in real-time. Partial least squares regression (PLSR) was developed as the preferred chemometrics quantitative analysis of the critical intermediate qualities: the danshensu (DSS, (R)-3, 4-dihydroxyphenyllactic acid), protocatechuic aldehyde (PA), rosmarinic acid (RA), and salvianolic acid B (SAB) concentrations. Optimized PLSR models were successfully built and used for on-line detecting of the concentrations of DSS, PA, RA, and SAB of water precipitation during Danhong injection. Besides, the information of DSS, PA, RA, and SAB concentrations would be instantly fed back to site technical personnel for control and adjustment timely. The verification experiments determined that the predicted values agreed with the actual homologic value.

Highlights

Danshen and Honghua are two traditional herbal medicines commonly used in China for the treatment of cardiovascular diseases
The present study has demonstrated that NIR spectroscopy provided us with important advantages to determine the concentrations of DSS, protocatechuic aldehyde (PA), rosmarinic acid (RA), and salvianolic acid B (SAB) in a quantitative and nondestructive manner and in an extremely short time
Based on the High Performance Liquid Chromatography (HPLC) reference method, the combination of NIR spectroscopy with Partial least squares regression (PLSR) was investigated for on-line detection of the compositions of Danhong injection during the water precipitation process

Summary

Introduction

Danshen and Honghua are two traditional herbal medicines commonly used in China for the treatment of cardiovascular diseases. Danhong injections are applied to activate circulation of the blood and resolve stasis to promote regeneration [1], and the formula is applied extensively to tens of millions of patients for the prevention and treatment of coronary artery disease [2] in clinics in China. The watersoluble ingredients, such as phenolic acids, are the medicinal components in the Danhong injection and promote blood circulation and resolve stasis to promote regeneration [4]. The industrial production of the Danhong injection uses alcohol precipitation initially to purify the crude extract. This process removes some macromolecular substances such as proteins, polysaccharides, tannins, and pigments [5]. Certain fat-soluble compounds, primarily tanshinones, are removed via subsequent water precipitations [6]

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