Near-infrared spectroscopic determination of human serum albumin, γ-globulin, and glucose in a control serum solution with searching combination moving window partial least squares

Abstract

Near-infrared (NIR) spectra in the region of 5000–4000 cm −1 with a chemometric method called searching combination moving window partial least squares (SCMWPLS) were employed to determine the concentrations of human serum albumin (HSA), γ-globulin, and glucose contained in the control serum IIB (CS IIB) solutions with various concentrations. SCMWPLS is proposed to search for the optimized combinations of informative regions, which are spectral intervals, considered containing useful information for building partial least squares (PLS) models. The informative regions can easily be found by moving window partial least squares regression (MWPLSR) method. PLS calibration models using the regions obtained by SCMWPLS were developed for HSA, γ-globulin, and glucose. These models showed good prediction with the smallest root mean square error of predictions (RMSEP), the relatively small number of PLS factors, and the highest correlation coefficients among the results achieved by using whole region and MWPLSR methods. The RMSEP values of HSA, γ-globulin, and glucose yielded by SCMWPLS were 0.0303, 0.0327, and 0.0195 g/dl, respectively. These results prove that SCMWPLS can be successfully applied to determine simultaneously the concentrations of HSA, γ-globulin, and glucose in complicated biological fluids such as CS IIB solutions by using NIR spectroscopy.