Abstract
We demonstrate optical resolution photoacoustic microscopy (OR-PAM) of lipid-rich tissue using a multi-wavelength pulsed laser based on nonlinear fiber optics. 1047nm laser pulses are converted to 1098, 1153, 1215, and 1270nm pulses via stimulated Raman scattering in a graded-index multimode fiber. Multispectral PAM of a lipid phantom is demonstrated with our low-cost and simple technique.
Highlights
Photoacoustic microscopy (PAM) [1,2] of lipid-rich tissue, such as atherosclerotic plaques [3,4,5,6] or myelinated peripheral nerves [7,8], typically requires near-infrared laser wavelengths near 1210 nm or 1720 nm
We demonstrated the first application of both techniques for PAM, where the desired wavelength is selected by a band-pass filter [11,12]
The laser pulses are coupled into 95 m of a graded-index multimode fiber (GIMF) with a 50 mm core diameter and 0.22 numerical aperture (GIF-50C, Thorlabs)
Summary
Photoacoustic microscopy (PAM) [1,2] of lipid-rich tissue, such as atherosclerotic plaques [3,4,5,6] or myelinated peripheral nerves [7,8], typically requires near-infrared laser wavelengths near 1210 nm or 1720 nm. These wavelengths correspond to the second and first overtone optical absorption of CÀÀH bonds in lipids [7]. The very high cost and slow wavelength tuning are major drawbacks for practical applications, where multiple wavelengths are preferred to distinguish lipids from surrounding tissue
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