Abstract
In site imaging of telomerase is essential for cancer diagnosis and treatment since telomerase is a crucial tumor marker and can immortalize the tumor. Precise probes designed for telomerase detection and imaging would be of value for cancer diagnosis. In this work, a near infrared (NIR) light-activated probe for spatiotemporally controlled in site imaging of telomerase was created based on upconversion nanoparticles (UCNPs) and a double-stranded DNA. In this probe, the UCNPs carried the double-stranded DNA enter cells autonomously and can convert NIR light irradiation into UV light to control the opening of the probe at the appropriate time and sites. The double-stranded DNA contains a photo-cleavable linker (PC linker) that can be cleaved by UV light and can be elongated by telomerase to reinstate the fluorescence of the probe. Confocal fluorescence imaging and in vivo imaging demonstrate the successful use of this probe for telomerase imaging in many tumor cells such as HeLa cells and nude mice. These results suggest that this probe has a promising future in precise biological molecular imaging for cancer diagnosis and treatment.
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