Near-infrared carbon dots-based fluorescence turn on aptasensor for determination of carcinoembryonic antigen in pleural effusion

Abstract

Early detection of carcinoembryonic antigen (CEA) is of great significance for the screening, diagnosis, monitoring and prognosis analysis of lung cancer. Herein, a novel fluorescence aptasensor with high signal-noise ratio (SNR) was constructed to achieve highly-sensitive detection of CEA relied upon the fluorescence resonance energy transfer (FRET) between near-infrared carbon dots (NIR-CDs) and gold nanorods (AuNRs). Initially, AuNRs@SiO2-Aptamer and NIR-CDs-DNA probe were prepared via the covalent bonding reaction between their corresponding carboxyl and amino groups, respectively. After DNA hybridization, the aptasensor was formed, meanwhile, the fluorescence of NIR-CDs was quenched by AuNRs@SiO2. Once CEA encountered the aptasensor, it would selectively combine with CEA aptamer to unwind the preformed DNA double-strand architecture thereby resulting in the NIR-CDs-DNA detach from the surface of AuNRs@SiO2. The attendant fluorescence recovery of NIR-CDs was linearly correlated with the concentration of CEA. According to this relationship, the NIR-CDs based “turn on” sensing system was constructed and exhibited prominent responses toward CEA in the concentration range of 0.1–5000 pg/mL and a relatively low detection limit (0.02 pg/mL). Moreover, it displayed high specificity against other biomarkers or proteins, good reproducibility and acceptable accuracy regarding human pleural effusion samples.